FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24727618
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC91250.019.3836081
GATGAAA88150.019.0538961
GAATGAA72850.018.9460891
ATGCGAT78900.018.8185671
GGCGACT86000.018.5831681
ACTGATG61300.018.1886561
AGCTTGA63600.018.1712061
TCTATTG97800.018.085421
TGATTTC76550.018.0734751
CAATGGA93300.018.0256188
TTTACTG89950.017.999611
GCGCGAT55450.017.824871
TGCACCT108400.017.7282771
GGGACCT64300.017.6481551
GTAACTG79600.017.6160281
GATGCTA72900.017.574261
GGTATTG98550.017.5568871
CTCGAAA62550.017.490841
TCTAACT83150.017.4612798
AGAAACC74600.017.442568
Produced by FastQC (version 0.11.5)