FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9255501
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACT1156850.021.8583951
AGAGTGG821700.021.8457371
GCAATTG972200.021.8132042
GTTCTCG777500.021.8100261
CTAACTT726650.021.7784941
TCTACTT1162550.021.737032
AGTGAGG639000.021.727311
CTCGGTA762400.021.7201734
GTACCCA763200.021.694298
GGCAATT984750.021.6882481
CGGTACC762800.021.6871536
AGTTATC490200.021.6703323
TCGGTAC763200.021.667145
GGTACCC764050.021.6615187
CTACTTC1169650.021.6578713
GCAGTTA496900.021.6490331
ACTTCAT1173200.021.6409765
ACCCAAT764500.021.63725710
GAGTGGG819600.021.6332742
TACCCAA765250.021.6318639
Produced by FastQC (version 0.11.5)