FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9255501
Sequences flagged as poor quality0
Sequence length91
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT184426119.926106647279276No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT2134942.306671459492036No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA807740.8727134273984737No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA772200.834314641638524No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG697340.7534330124322821No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT592840.6405271848601173No Hit
GCGGTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT501000.5412997092215753No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC384220.4151260963615044No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT334650.36156875786626785No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC300980.3251903921786622No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT253230.27359945182870166No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC237070.25613956500031715No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG224060.2420830595772179No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA212930.23005777861187632No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG185490.20041054503694614No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAGAGAAGTTATT163530.1766841146686711No Hit
GCGTTATCCACCTTCCACTGTACGTTGGCCTCTCTGGGATAGAAGTTATT160490.17339958150293538No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA160120.17299981924263202No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG154810.16726269058800813No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCGGGGATAGAAGTTATT146380.15815459368433973No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATCGAAGTTATT140230.15150989665497308No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC140140.15141265718625063No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT132550.143212128657325No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT123620.13356381248297633No Hit
GCGTTATCCACCTTCCACTGTACTGTGGCCTCTCTGGGATAGAAGTTATT122620.1324833739416159No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC118220.12772944435963002No Hit
GCGTGATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT114900.1241423884023134No Hit
CCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGG114030.12320240687132983No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG112610.12166818414259802No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGCTAGAAGTTATT108890.1176489527687372No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC103290.11159849693711879No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG101750.10993462158342374No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGGTATT100880.10899464005244017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT2288250.084.7392651
GCGGTAT98050.083.7367861
CGTTATC2313800.083.616192
CGGTATC98300.082.6159442
GCGTTAG9650.081.030261
GCGTGAT29150.080.3285141
TTATCCA2418600.079.97374
CGTGATC29050.079.581022
CACGCTG104400.078.8878251
CGTTAGC9700.078.860132
ATCCACC2588300.078.6872256
GTTATCC2514050.077.35323
TATCCAC2631000.076.516945
TCCACCT2673150.076.440757
CACCTTC2687200.075.9967969
CCACCTT2775050.073.589438
GGTATCC117200.071.795013
GCGTTAA6000.070.11981
GGGTTAT18350.068.7823641
CGTTCTC16500.068.767632
Produced by FastQC (version 0.11.5)