FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25604761
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC93850.019.1168651
GATGAAA92400.018.9641971
GGCGACT88750.018.8015751
GAATGAA80900.018.5307061
GCGCGAT55800.018.1869581
ATGCGAT81150.018.1616551
GGGACCT67400.018.1596831
TCTATTG99300.018.134161
CAATGGA94700.017.968228
TGATTTC74500.017.9212341
TTTACTG93450.017.8441851
TGCACCT114150.017.7613951
GTAACTG84950.017.7249931
GGTATTG102700.017.6195471
GATGCTA81150.017.578421
CTGATCC92650.017.546821
TGACTAG66150.017.5044821
ACTGATG63400.017.4825021
AGAAACC73550.017.4822228
CCTTACG67900.017.4261741
Produced by FastQC (version 0.11.5)