FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29931104
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC107100.019.2687631
GATGAAA108150.019.000271
GGCGACT104150.018.8528751
GAATGAA94700.018.4794561
ATGCGAT98450.018.446341
TCTATTG118600.018.1891921
TTTACTG108350.018.0611271
GGGACCT76450.017.9815431
CAATGGA112150.017.879348
AGCTTGA78600.017.8677621
TGCACCT131200.017.7845971
GCGCGAT66050.017.7800751
TGATTTC90150.017.7761481
AGAAACC91150.017.7146858
GATGCTA93250.017.704531
GTAACTG98250.017.57651
CTGATCC104350.017.5088521
CCTACCA99450.017.4529531
AGAAAGG71750.017.3688910
ACTGATG78150.017.308721
Produced by FastQC (version 0.11.5)