FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11081755
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC41550.019.3105031
GATGAAA40050.018.6047251
ATGCGAT36050.018.5929851
GATGCTA33150.018.526261
GGCGACT39000.018.4283161
GCGCGAT24950.018.2186661
TCTATTG42000.018.1078071
GGGACCT27900.017.9886151
ACTGATG28250.017.9605461
TTTACTG36600.017.9226511
GGTATTG45900.017.9120391
GAATGAA32800.017.8179511
TGCACCT49050.017.7939051
TGATTTC32550.017.7857361
CAATGGA42800.017.7068798
GACTAAC30700.017.6385881
CCTACCA36100.017.4696621
CCACTAC33600.017.3937151
CCGGTAG20750.017.3446981
AGCTTGA29550.017.3193871
Produced by FastQC (version 0.11.5)