FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20604077
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT70750.018.922181
GCGCGAT60100.018.6328961
TGACTAG62000.018.3280241
CAATGGA61000.018.248818
CACCGAA60250.018.202128
AAACCTG70900.018.152931
AGAAACC62950.018.0679428
TGAGCCG57700.017.8446121
ACTGAAC50000.017.820591
CTGATAG64350.017.7099881
GGGAATG51750.017.6643521
ACCCACT72600.017.65211
AGACTAG63850.017.6237628
ATCGAGT67650.017.5778161
TTGACTT77900.017.566691
CTGAAAC46300.017.5340581
CGGACTG53400.017.4893441
CGCTGGA53200.017.4517041
CGCTATC56500.017.4448261
ATAACGC66500.017.4186211
Produced by FastQC (version 0.11.5)