FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20651564
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT72800.018.736741
GGGAATG50500.018.406411
TGACTAG60350.018.3003831
GCGCGAT62500.018.2692661
AGACTAG62500.018.074968
CACCGAA62150.017.999768
CCCTCCT74200.017.9977631
TGAGCCG58600.017.9458481
AGAAACC62200.017.8791838
TTGACTT76850.017.8781381
AAACCTG74750.017.8064731
ACTGAAC49250.017.7567961
CTTAACT50650.017.7437881
CTGATAG66350.017.6899761
TTGGAAC60400.017.6660121
ATCGAGT68000.017.6651551
AGCTTGA51600.017.6089741
CGGACTG51200.017.5961511
GGGACCT50700.017.5310171
ACTGATG50150.017.4819981
Produced by FastQC (version 0.11.5)