FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10700191
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT36000.019.0060351
TGAGCCG32650.018.9346281
AAACCTG33700.018.932231
TGACTAG31500.018.2639541
AGACTAG31500.018.2631878
CACCGAA30450.018.206598
GCGCGAT32000.018.150461
AGAAACC35100.017.9569648
AGCTTGA26600.017.947821
ATCGAGT36350.017.9151731
CTTAACT26900.017.9112321
TTGACTT39800.017.911
CGGACTG27850.017.8927331
TTGGAAC33650.017.81621
TGATTTC29850.017.7625941
CCCTCCT40200.017.7044281
GGGAATG26850.017.698771
TGCACCT44450.017.6944851
CTAATGG36000.017.6921121
TAGTTGG34250.017.664681
Produced by FastQC (version 0.11.5)