FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10714447
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT38500.018.9715251
GCGCGAT29750.018.7093371
CACCGAA33450.018.546928
TAGTTGG33100.018.3777371
TGACTAG30650.018.3394091
ATCGAGT35100.018.2707481
GGGAATG25950.018.2698421
ACTGAAC24500.018.1388681
TGAGCCG31250.017.9168911
GATGAAA23650.017.8605561
CCCTCCT40000.017.8475911
AAACCTG37250.017.8068031
AGAAACC31950.017.799538
CTGAAAC25550.017.737861
AGACTAG32500.017.701388
TTGACTT38650.017.6171671
ACCCACT36950.017.5941351
CTTAACT28900.017.5848641
TGATTTC31850.017.5102921
CAATGGA33900.017.489528
Produced by FastQC (version 0.11.5)