FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11018279
Sequences flagged as poor quality0
Sequence length91
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC128183111.633677092402543No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC7783287.063970698146235No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC6181215.609959595323372No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC3671253.3319631859022625No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC1278051.1599361388470921No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT789900.7168996174447934No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC625330.5675387236064725No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG541510.4914651371598051No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA521310.4731319655274658No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT471540.42796157185709305No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT385930.3502634122806293No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG333580.30275145510474No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTT319420.289900083307021No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGG314720.28563444436286284No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG314330.2852804870887731No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATG311660.2828572411353896No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA284140.25788056374321255No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC279110.2533154224902092No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTT277870.25219001987515477No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTT267650.24291452412849593No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATTC266150.24155314999738162No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTC263110.23879409842498997No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTG258340.23446492868804647No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG248850.22585196835186327No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAA239490.21735699377371004No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG239070.21697580901699803No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG238280.21625881864127786No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA225680.20482327593991764No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAA221540.20106588333804218No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC219830.19951391682857186No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG219170.19891491221088156No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG217150.19708159504764766No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGT216710.19668225863585412No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG208290.18904041184653247No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGGGTG189360.17185987031186994No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC188450.1710339700056606No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGA187180.16988133990798382No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTC183160.16623285723659748No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTC182150.1653161986549805No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCAATC180540.16385499042091783No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG165120.14986006435306276No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCAGC163130.1480539746724511No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGACTG156110.14168274373883616No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCCGG150170.13629170217962353No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGACCACGTTTTTCAGGTCC149650.13581975914750388No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTGG148750.1350029346688353No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTT142260.12911272259488074No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGAATA134330.12191559135505643No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGA132650.12039085232820841No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGAAGAG127020.1152811614227594No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAA125100.11353860253493309No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTAAGATCC125010.11345692008706623No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGATTC123820.11237689660971555No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGTGGTAAA120780.10961784503732389No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGACTTG120730.10957246589962007No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTA114710.10410881772008133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTCT1281300.081.049111
TCTGATG1336550.079.438581
GTCTCTC1546800.067.2098542
TCTCTCA1589150.065.3706053
CTCTCAG1864000.055.888924
TCAGCTG1874950.055.340387
AGCTGGT1878150.055.120139
TCTCAGC1899550.054.82735
CAGCTGG1907350.054.482768
CTCAGCT1909200.054.4166156
CTGATGG3415450.051.264041
TGATGGC3510100.049.8911252
TCCGATC11150.049.170856
CTTCCGA11350.048.3044054
GATGGCT3684900.047.4114233
TGGCTCA3722000.047.082715
GGCTCAA3757600.046.854936
ATGGCTC3737350.046.760834
GCTCAAA3766600.046.679797
CTCAAAC3777650.046.5522468
Produced by FastQC (version 0.11.5)