FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11041492
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT32600.018.727421
ATGCGAT38000.018.642531
TGACTAG31500.018.2988321
TGATTTC34300.018.216161
TGAGCCG32900.018.1888661
AGACTAG35800.018.1282488
CAATGGA33600.018.0711868
CACCGAA35850.018.0416018
ACCCACT40100.017.9131281
ATCGAGT37300.017.9012131
CCTACCA37400.017.853351
AGAAACC33400.017.8500618
GGGACCT29500.017.8241351
AAACCTG39750.017.7941191
TAGTTGG36350.017.673031
ACTGAAC25250.017.6439671
TTGACTT41550.017.6056961
ACTGATG27900.017.5452041
CGGACTG28400.017.5074421
TTGGAAC34000.017.4709871
Produced by FastQC (version 0.11.5)