FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14181062
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC45000.017.4502471
CAATGGA50450.017.3425778
GGGAATG47350.017.0912171
ATGCGAT39750.017.0371111
GGCGACT39700.016.957781
CTCGAAA37650.016.8980481
GACCTGG43550.016.8138581
CTGCCTA43550.016.6530691
TATGCCC47300.016.3902281
CAATGAA29000.016.3780578
GGGAGAT37450.016.3472231
TTTATGC34850.016.3038431
CCGGGAT36450.016.3017161
GATGAAA35450.016.1972051
GGATTAC45250.016.1379621
CATGACA45200.016.089421
CGCTTCA36400.016.0767751
TGCGGGT35400.016.0505351
GCTGCAG49300.016.0499611
CCTACCA43200.016.0470031
Produced by FastQC (version 0.11.5)