FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14076393
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC44850.017.6188811
CAATGGA51300.017.133518
GATGAAA37450.017.093931
CTGCCTA42750.017.0570961
GCGCGAT22800.017.0221
ATGCGAT37100.016.9046961
GGATTAC46150.016.8408131
GGGAATG48050.016.757771
CTCGAAA33350.016.6460381
ACTGATG31450.016.4749011
TGAGCAT39550.016.4644891
CCGGGAT35550.016.4600051
GGCGACT37950.016.4206371
CATGACA48550.016.3997631
GACCTGG42400.016.3014371
TGCCAAA39950.016.27461
CGAGCAC43200.016.2542551
AGCTTGA33350.016.1961461
GGGAGAT35550.016.15051
CTTGGCT51650.016.1320061