FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12652684
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC42700.017.2645241
CTGCCTA39000.017.1327421
GATGAAA34000.016.9456631
GCGCGAT19900.016.8888931
CAATGGA46900.016.8858598
GGCGACT33900.016.8776251
ATGCGAT30950.016.838061
GGGAATG40950.016.8054261
GGATTAC43200.016.7868521
CTCGAAA31200.016.703141
CCGGGAT31550.016.5812531
ACTGATG29200.016.5797331
GACCTGG38750.016.5463181
AGCTTGA29400.016.4669461
TGAGCAT34750.016.3784391
CCTACCA38950.016.3586331
CATGACA43200.016.3237671
CATGGCG39800.016.2857191
TTTACTG36950.016.0258941
CGCTGGA31700.015.9979271