FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12604708
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA44900.017.3709148
ACTGAAC39550.017.2991711
GGCGACT33500.017.1388931
GATGAAA33500.016.98961
GGGAATG45100.016.700681
ACTGATG27200.016.6956231
CGCTTCA31100.016.6603871
CTCGAAA33550.016.6363261
CCGGGAT30250.016.632531
ATGCGAT33050.016.5853561
GACCTGG37400.016.5819571
CTGCCTA40500.016.5476171
TGAGCAT33750.016.3006361
CCACTAC36200.016.1921581
TGCCAAA36500.016.1686921
GGATTAC43000.016.1438351
ACCCACT38150.016.1248721
CGGACTG26200.016.0729851
GGGAGAT34450.016.0565261
CGCTGGA31600.016.0485781
Produced by FastQC (version 0.11.5)