FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17031859
Sequences flagged as poor quality0
Sequence length28
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT50500.018.5817361
GGATTAC75600.018.4658131
GGCGACT39750.018.3763641
TGACAAC66750.018.310131
GACTAAC40150.018.001491
CCGGTAG49450.017.9751991
CCACTAC54200.017.8612561
TGATTTC49200.017.8429371
CTCGAAA52900.017.78031
AAACCTG47550.017.7448981
GCGCGAT42800.017.709411
ATAGACC48150.017.6608581
AGAATGT42250.017.6517188
ACTGCTC60900.017.594231
CAATGGA52550.017.5829038
ACTGATG36950.017.565731
GGAACTT51900.017.5505851
GCCGAAG27300.017.4870363
CACCGAA40300.017.4686348
TCTATTG59000.017.4336471
Produced by FastQC (version 0.11.5)