FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17575981
Sequences flagged as poor quality0
Sequence length28
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT51750.019.1324461
TGACAAC66150.018.7260821
CCGGTAG49750.018.5527041
GGATTAC77650.018.5170781
GCCGAAG27200.018.2390733
TGATTTC54350.018.2171861
CCACTAC53950.018.2095151
TGGACGC50600.018.0236321
CTCGAAA54800.017.9872931
GCGCGAT48550.017.9462741
AGAATGT41850.017.9255688
ACTGATG39600.017.862991
ACTGAAC50650.017.8103621
TATGCCC55450.017.7367611
AAACCTG48600.017.7014481
TGACGGC38750.017.6302411
ACGAGCC51950.017.5976141
GATCTAG52550.017.5851041
GGCGACT41000.017.4945221
CATGACA58750.017.4333211
Produced by FastQC (version 0.11.5)