FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17302424
Sequences flagged as poor quality0
Sequence length28
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT50500.019.0600511
GGATTAC76000.018.9032691
CCGGTAG46800.018.3809621
GGCGACT40700.018.2438581
ACTGATG39200.018.1562251
TGACAAC69050.018.0976431
GCCGAAG28200.018.0604763
GCGCGAT42950.017.9284291
TGATTTC52900.017.9249921
GGAACTT50950.017.8337731
ACGAGCC48200.017.7786081
ACTGAAC53250.017.7658581
TGGACGC47900.017.72921
AGAATGT46250.017.6236678
CAATGGA55000.017.6198838
GACTAAC39100.017.6118491
CTCGAAA49150.017.5916421
TGACGGC37850.017.5831571
CGCTATC51300.017.4547791
ACTGCTC62050.017.4445861
Produced by FastQC (version 0.11.5)