FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15814330
Sequences flagged as poor quality0
Sequence length28
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT45150.019.2969441
GGATTAC66700.018.6698881
TGACAAC57200.018.3858171
CCGGTAG44100.018.1349741
GACTAAC36800.018.085421
GCCGAAG26500.017.9733963
TGATTTC45000.017.9433961
GCGCGAT39650.017.9229871
ACTGCTC56800.017.8374331
CTCGAAA46950.017.8307721
TGAGCCG37300.017.8134981
ACTGATG34000.017.795281
GGCGACT35150.017.7451151
TCTATTG54050.017.7273391
CGCTATC46200.017.7154431
ACTGAAC45150.017.6888641
CCACTAC50450.017.6185951
TGGACGC43600.017.5860121
CAATGGA48950.017.4827968
GATCTAG47950.017.4818521
Produced by FastQC (version 0.11.5)