FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13108537
Sequences flagged as poor quality0
Sequence length28
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT36600.019.176311
GGATTAC55900.018.911981
TGACAAC50100.018.8397451
TGATTTC39350.018.367331
CTCGAAA40250.018.2846091
CCGGTAG35350.018.2672881
GCGCGAT33350.018.1752831
TGACTAG31650.018.1087911
TCTATTG45750.018.0581931
GACTAAC28100.017.9301781
AAACCTG35500.017.880221
GCCGAAG20800.017.875123
ACTGAAC38350.017.8135991
CGCTATC37550.017.8122621
AGAATGT33650.017.7826398
ACTGCTC49150.017.7042871
GATCTAG37550.017.665781
CAATGGA44150.017.6394468
CTGATCC46000.017.5295831
AGCTTGA29000.017.525461
Produced by FastQC (version 0.11.5)