FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21879794
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC73650.018.6116311
GGGAATG66450.018.5405651
GGCTCGA61800.018.2074991
TTGGAAC62700.018.1919841
CTGAAAC55650.018.1620711
TCTGGAA88850.018.153821
GGCGACT57750.018.1307771
TTGAACG59500.018.1156371
CCTACCA82050.018.074941
CGGACTG67150.018.0685581
ACTGATG60650.017.989981
GCGCGAT52350.017.9399091
TGACTAG44750.017.9112631
CGCTTCA64350.017.8623981
CTCGAAA63750.017.840541
GATGAAA53050.017.806961
CTGCCTA72400.017.7163891
TACGGGC84550.017.6967581
TGACGGC52200.017.696171
TGAGCCG61950.017.6124671
Produced by FastQC (version 0.11.5)