FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21846865
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACT56650.018.846721
CTGAAAC57100.018.7752951
GGCTCGA62600.018.4620081
ACTGATG62700.018.4150091
TCTGGAA87150.018.3889921
TTGGAAC66550.018.3420331
TTGAACG58550.018.2915251
CCTACCA85800.018.2678171
GGGAATG72500.018.1879081
CTCGAAA62200.018.0321621
GGATTAC74350.017.9130021
GAATGAA62800.017.912461
GCGCGAT49900.017.9109821
CGGACTG63200.017.886171
CGCTTCA63550.017.8223021
CTGCCTA72350.017.7996431
CAATGGA77100.017.7473148
GATGCTA70300.017.66111
AAACCTG61850.017.5825231
AGCTGAA51200.017.4227858
Produced by FastQC (version 0.11.5)