FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20900635
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAA82250.018.607641
TTGAACG53950.018.5721551
CTGAAAC54400.018.560211
GGCGACT56350.018.4455011
GGGAATG66300.018.3510591
GGCTCGA59900.018.2897871
TTGGAAC62100.018.2801361
GGATTAC65700.018.2672651
CTCGAAA57450.018.0731581
ACTGATG58850.017.8490161
GACTAAC43750.017.8435211
CCTACCA80100.017.8149621
CAATGGA71150.017.792838
GATGCTA70000.017.7900411
CGCTTCA57750.017.750481
ACTGAAC44600.017.7256411
CGGACTG64500.017.6170181
GAATGAA57150.017.5707781
CTGCCTA68250.017.5524861
GCGCGAT53900.017.5271341
Produced by FastQC (version 0.11.5)