FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20774968
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAA83250.018.6682221
GCGCGAT50600.018.5763281
GGATTAC68800.018.4296281
TTGAACG55700.018.416761
GGGAATG64350.018.2844411
GGCTCGA58400.018.2626361
ACTGATG58650.018.1472571
TTGGAAC61100.018.1041181
CTGAAAC54750.018.072911
GACTAAC44250.018.0582581
CCTACCA80850.017.9563181
CTCGAAA60600.017.8720761
ACCCACT73650.017.7988341
TGAGCCG57700.017.7783991
CTGCCTA70450.017.7636411
CGGACTG62050.017.7559871
CAATGGA74950.017.7427838
CGCTTCA62700.017.7123491
GGCGACT57150.017.6991251
TGACTAG43100.017.6207491
Produced by FastQC (version 0.11.5)