FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12128644
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAA49200.018.8200931
GATGCTA39950.018.4925421
CTGAAAC34000.018.4904751
CTCGAAA33550.018.4103151
GGCTCGA35550.018.3656351
GGCGACT30750.018.3322471
GGATTAC42050.018.328331
TTGAACG33250.018.2784041
GGGAATG37050.018.2758581
ACTGATG34350.018.173861
TTGGAAC36750.018.1554131
CAATGGA44750.018.0650488
CTGCCTA41450.017.9295771
TGACGGC27950.017.884011
ACTGAAC26350.017.883561
GCGCGAT29450.017.8329811
ACCCACT43650.017.6817261
TGACTAG26100.017.6752011
AGCTGAA29200.017.6658618
CCTACCA44450.017.6607281
Produced by FastQC (version 0.11.5)