FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12052383
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGAAC34250.018.4786451
TCTGGAA47200.018.4691141
ACTGATG32300.018.367461
GGCTCGA33150.018.3613431
CTCGAAA33700.018.3556291
GGATTAC39100.018.3541261
GGGAATG41350.018.2870671
CCTACCA49600.018.2189921
GCGCGAT27250.018.1764411
CGGACTG34550.018.0314751
CTGAAAC31350.017.9409851
GGCGACT31750.017.9229581
CTGCCTA38900.017.8259961
TTGACTT39300.017.7565921
ACCCACT42750.017.7139421
ACTGAAC24200.017.6473391
GAATGAA34850.017.6235891
TTGAACG32300.017.5155371
TACGGGC44200.017.5063631
CAATGGA43000.017.4965068
Produced by FastQC (version 0.11.5)