FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12173199
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA39500.019.1605851
TGACTAG39700.018.925511
ACTGAAC35950.018.9106731
ATAGACC38900.018.7208611
GGGACCT31100.018.4640731
GGCGACT39100.018.2312161
TTGGAAC42000.018.229611
CTCGAAA30900.018.1919751
ACTGATG38100.018.1900121
TACACGA40250.018.1749481
TGATTTC37450.018.1238611
ATGCGAT42250.018.121741
TCTGGAA45500.018.060371
CGATTGA41150.017.964571
AGCAAAG32100.017.9565983
GACGTGC55550.017.902011
CGCTATC42050.017.815521
GGTATTG45550.017.7990381
GGAACTT36800.017.7265531
AGCAACA45550.017.7257843
Produced by FastQC (version 0.11.5)