FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28762310
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA92450.018.931231
ACTGAAC89600.018.882691
GGCGACT89650.018.5899371
TGACTAG87900.018.5220221
TCTGGAA113950.018.3521
TACACGA94750.018.3207551
ATGCGAT95550.018.2824921
CTCGAAA69450.018.1204661
TTGGAAC93700.018.0212271
CGCTTCA84050.017.9569241
CGATTGA98050.017.9397531
ACTGATG84100.017.9200881
TGATTTC88700.017.858891
AAACCTG93500.017.8362331
GAACCTA88250.017.8253021
ATAGACC97050.017.7165451
CGCTATC95650.017.6768321
GACGTGC132600.017.6457271
CTAAGAC90300.017.6277281
GGGACCT71550.017.5886271
Produced by FastQC (version 0.11.5)