FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29229100
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC89550.019.163251
TGACTAG97300.018.914431
GAATGAA93350.018.8781031
GGCGACT90850.018.3441621
ACTGATG84750.018.2626821
TACACGA95350.018.216781
TTGGAAC93250.018.214141
TGATTTC85250.018.155571
TCTGGAA109500.018.133151
CGATTGA99850.018.1229131
AAACCTG94350.018.1067181
GGGACCT75250.018.0100291
ATAGACC99750.017.9094921
CTCGAAA70900.017.9048121
CGCTTCA86700.017.8646181
AGAAACC81000.017.844348
GATTCAG116050.017.8301031
CGGACTG71500.017.8161011
ATGCGAT101800.017.6893181
GGAACTT87250.017.6763571
Produced by FastQC (version 0.11.5)