FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17151215
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA55800.019.1226271
ACTGAAC53000.018.9083041
ATAGACC60650.018.6635671
GGCGACT51700.018.575211
TGACTAG54350.018.41841
TTGGAAC54050.018.3374581
TACACGA60500.018.1098161
CTCGAAA41350.018.0369971
ACTGATG50700.018.0303081
ATGCGAT61950.017.9522911
GGGACCT43750.017.8521441
GAACCTA54200.017.8401971
GATTCAG69500.017.8222961
CGATTGA56600.017.7833981
GTAACTG53100.017.7747211
CAATGGA52150.017.7601918
AGCAACA61700.017.667943
TCTGGAA61900.017.646911
CTAAGAC55400.017.6324751
GGTATTG60150.017.57511
Produced by FastQC (version 0.11.5)