FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17471752
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC55850.019.1253031
GAATGAA55100.019.0063041
GGCGACT53400.018.7873651
TGACTAG57900.018.5051541
TTGGAAC57500.018.4234391
TCTGGAA66000.018.4008311
TACACGA56750.018.3373931
ACTGATG53400.018.210561
TGATTTC54400.018.0982441
CGCTATC59100.017.9805071
GGAACTT51300.017.9696221
ATGCGAT55550.017.9612061
CGCTTCA52400.017.9282911
GATGAAA44650.017.8865851
ATAGACC59000.017.8245321
CTCGAAA43650.017.8175281
CTAAGAC55550.017.7631781
GGTATTG61550.017.7473491
GGGACCT47350.017.726251
TGCTACC58450.017.6723121
Produced by FastQC (version 0.11.5)