FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7313968
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG24400.019.2508031
ACTGAAC24350.019.0192741
GAATGAA23200.018.8714751
CTCGAAA17650.018.6976621
TTGGAAC23900.018.5028631
CCTACCA25750.018.4124161
ATGCGAT24800.018.4080241
TACACGA21850.018.376051
GGCGACT21350.018.2396371
TGCTACC23350.018.2320231
GATTCAG27500.018.0807531
GGAACTT23500.018.0688361
ATAGACC22500.017.9918611
CCGGGAT16850.017.953271
ACTGATG21750.017.9041941
GACGTGC32150.017.8607751
CGCTATC25900.017.8385811
ACGAGGA20000.017.8207421
CGATTGA24000.017.7840731
GTAACTG22000.017.7507381
Produced by FastQC (version 0.11.5)