FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7428229
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC22300.019.1889151
GAATGAA26150.019.0139541
TGACTAG25700.018.8760531
GGCGACT22200.018.7798421
TACACGA23700.018.7052041
TGATTTC23600.018.6446271
AAACCTG22400.018.4157311
TCTGGAA29200.018.3087671
ATAGACC23300.018.2709351
ATGCGAT23050.018.230481
TTGGAAC23300.018.2237221
GGGACCT18600.018.0973191
CGATTGA26000.018.0659281
ACTGATG20500.018.0298081
GGAACTT22050.018.0096111
GTAACTG23400.017.9578021
CAATGGA23000.017.8868928
CTAAGAC23200.017.8755361
CCTACCA25250.017.8183561
CTGCGGA14950.017.8065551
Produced by FastQC (version 0.11.5)