FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14010440
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT56450.019.5690631
ACTGAAC50750.019.273741
GAATAAG58250.019.2665751
AGCTTGA46750.019.0164761
GTAACTG62450.018.8517481
GATGCTA54150.018.7950531
AGAATGT53850.018.7718738
ACTGATG49750.018.7543951
GAATGAA45650.018.6552241
GACTAAC43600.018.623881
ACGATGT77100.018.594751
AGTGAAC34650.018.5707918
GCCGACA29450.018.5646213
TCTGGAA52800.018.4629081
TATGCCC57600.018.356981
GCATGCG63350.018.3234021
TGACTAG48000.018.269111
ACGAGGA40100.018.2464141
CGCTTCA41850.018.2195661
TGATTTC42050.018.132911
Produced by FastQC (version 0.11.5)