FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13937597
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATAAG59200.019.3677881
GCGCGAT55050.019.2087941
ACTGAAC45650.019.042341
ACTGATG50100.018.9981961
AGCTTGA49450.018.9141431
GTAACTG56600.018.8966061
GAATGAA44200.018.8206021
GATGCTA53900.018.781621
AGAATGT53350.018.741448
AGTGAAC36950.018.7244768
TGACTAG47000.018.659261
TATGCCC60650.018.650751
GACTAAC41350.018.4679181
ACGAGGA43700.018.4064461
TCTGGAA50100.018.3173351
CTGCCTA58100.018.1625351
CGCTTCA45950.018.1277681
GCGCAAC55100.018.0530591
ACGATGT78650.018.0198481
CTGAAAC47100.017.9888671
Produced by FastQC (version 0.11.5)