FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18092628
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT71450.019.3727321
AGCTTGA69400.019.2632391
ACTGATG63700.019.155991
GATGCTA71400.018.9239881
ACTGAAC60600.018.864941
GAATAAG75450.018.8415151
GAATGAA59050.018.7079541
GTAACTG77150.018.6402681
GGCGACT43350.018.6302831
TCTGGAA66100.018.5603391
ACGATGT96850.018.5523571
ACGAGGA55650.018.4471381
AGAATGT72350.018.4111658
GACTAAC55150.018.3550171
GGCTCGA46100.018.3543031
CTGAAAC59200.018.2702431
TGATTTC60700.018.1812921
TGACTAG59150.018.1368711
CGCTTCA57100.018.075041
TATGCCC76550.018.067691
Produced by FastQC (version 0.11.5)