FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17969183
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT70500.019.369511
ACTGAAC61950.019.058751
GAATGAA58250.018.9848041
GACTAAC50150.018.8696371
GAATAAG74900.018.7752231
ACTGATG63750.018.7450081
TGATTTC56200.018.5417061
AGCTTGA60600.018.466491
GATGCTA71050.018.4297281
GTAACTG76300.018.3874571
GCGCAAC69700.018.3446481
GGCTCGA46950.018.3276671
ACGAGGA59250.018.2743851
AGTGAAC43100.018.2474778
ACGATGT97100.018.2222841
GGCGACT42600.018.2102361
TCTGGAA63400.018.2063241
AGAATGT69500.018.2006598
CTGCCTA75900.018.1944081
TATGCCC73750.018.1727771
Produced by FastQC (version 0.11.5)