FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18111658
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT73700.019.3479271
ACTGAAC57350.019.1658921
ACTGATG65500.019.0488471
AGCTTGA64100.019.035771
GAATGAA60900.018.861671
GATGCTA70250.018.7945481
GAATAAG74350.018.7496241
GTAACTG79600.018.6049671
TATGCCC77900.018.5448881
AGAATGT69600.018.474968
ACGAGGA56700.018.4153561
CTGAAAC62000.018.4028031
GACTAAC49150.018.356391
TGACTAG61550.018.2870861
ACGATGT101800.018.2548751
CGCTTCA58000.018.2112671
TCTGGAA64950.018.1937451
AGTGAAC48550.018.1023858
CTGCCTA74400.018.0863321
GGCTCGA45750.018.0371171
Produced by FastQC (version 0.11.5)