FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17992341
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT72850.019.1978191
AGCTTGA63850.019.1120191
ACTGAAC61900.019.0563621
ACTGATG61650.018.7766691
GAATGAA61550.018.6820341
TATGCCC77550.018.587691
GACTAAC54700.018.5874821
GATGCTA71750.018.5106321
ACGAGGA57100.018.4614281
ACGATGT100900.018.452071
GCATGCG77100.018.4392831
GTAACTG75500.018.436541
TCTGGAA65400.018.4235061
AGAATGT68700.018.3966458
GAATAAG75750.018.3756941
AGTGAAC47250.018.2744038
TGACTAG60900.018.2671071
TGATTTC58250.018.1536241
CTGAAAC60100.018.1440871
TGCACCT61350.018.0793111
Produced by FastQC (version 0.11.5)