FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9991977
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG26000.019.7019731
ACTGAAC34850.019.3770561
GGAACTT33900.018.7177161
CGCTGGA39000.018.6144811
CTCTACG39750.018.5681111
GGGACCT30300.018.5420461
CGTGAGC31900.018.4408381
AGCTTGA27200.018.4277631
GAACCTA35550.018.3757551
TACTTGT40950.018.3468111
GCGCGAT22800.018.1186771
TGATTTC25000.018.0224321
GGGAATG28450.017.8504331
CGGACTG25300.017.8087271
GAATGAA22250.017.7743851
GACTAAC27050.017.756171
ATAGACC32900.017.7129081
AGATATG28950.017.7039158
TTTACTG31800.017.7020651
CTGATCC29000.017.7018241
Produced by FastQC (version 0.11.5)