FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15426669
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC50800.019.1915281
ACTGATG41900.018.9299071
CGCTGGA60000.018.7825431
AGCTTGA48850.018.7624151
GGGACCT42850.018.6644761
GGGAATG41750.018.6021291
CGTGAGC48150.018.6004851
CTCTACG62600.018.4071831
TACTTGT62900.018.4069611
GGAACTT51700.018.2821271
AGATATG48150.018.0678838
TCTAACT58950.018.0413788
GGATTAC53400.018.0095561
CGGAGCT42150.017.9551561
CTGATAG39450.017.8995061
GAACCTA56200.017.8179421
TGACGGC42050.017.814471
GACTAAC38100.017.8108961
TGACTAG43800.017.8069341
GCGCGAT36150.017.7964971
Produced by FastQC (version 0.11.5)