FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12026659
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC44900.019.1124171
AGCTTGA36050.018.8845981
GGAACTT40300.018.7244911
GGGACCT36300.018.6938421
TACTTGT49350.018.5274431
ACTGATG32050.018.4917831
CGCTGGA49600.018.4562681
CTCTACG48000.018.3141251
CGGACTG33100.018.1714341
GGGAATG31250.018.119141
AGATATG36050.018.030568
GAACCTA40450.018.0286831
GGATTAC42800.017.965371
TGACTAG35300.017.8815211
CGTGAGC39950.017.868281
TGACGGC32000.017.6944751
TCTAACT43000.017.6740728
ATAGACC40600.017.6636091
GACTAAC32650.017.6121271
GATGAAA32300.017.598341
Produced by FastQC (version 0.11.5)