FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15222623
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG49150.019.2570081
GGAACTT49050.018.6680161
TTGGAAC53350.018.5042671
TTGAACG46750.018.5035381
ATCGAGT53400.018.486941
GAATGAA35300.018.4258121
GTAAATG56300.018.1306068
CGCTATC65300.018.1010991
TACACGA57850.018.0541361
TGACAAC54100.017.9832761
TCTAACT62750.017.8796568
TGACTAG44750.017.8794961
ACGATGT66300.017.877881
TGCACCT55500.017.8270951
GAACCTA50150.017.8196411
GACCTGG42550.017.7434641
GTAACTG49550.017.6800421
ATGCGAT37000.017.638711
TGATTTC40500.017.581791
GACTAAC36700.017.572981
Produced by FastQC (version 0.11.5)