FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15184690
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG49550.018.9237251
GTAAATG59200.018.6174728
GGAACTT51200.018.5073361
TTGGAAC51250.018.489281
ATCGAGT53150.018.4288161
TCTGGAA47300.018.264981
GAATGAA35550.018.2341881
CGCTATC64300.018.1257381
TTGAACG46850.018.1115441
TCTAACT64250.018.0957498
TACACGA58150.018.0365681
TGACAAC50050.018.0310271
GAACCTA51400.017.985681
AAACCTG42250.017.8693241
TGACTAG47600.017.8492951
CCGGGAT44900.017.7951221
ACTGAAC40150.017.7623391
CGATTGA42800.017.7425481
TGCACCT54900.017.7411441
GACCTGG45750.017.6810051
Produced by FastQC (version 0.11.5)