FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19515024
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACTT62050.018.9782541
ACTGATG59350.018.8217331
ATCGAGT66050.018.7453691
TTGGAAC64350.018.402551
CGCTATC80000.018.3931311
TTGAACG58550.018.3834171
TACACGA78650.018.1211321
GACCTGG57350.018.0772251
TGACTAG55750.018.0630251
TCTAACT77950.018.0338558
GTAAATG74450.018.0099668
TGACAAC68750.017.8651121
TCTGGAA61150.017.853751
CACGAGT56400.017.769923
GAACCTA64650.017.7553841
CCGGGAT59050.017.7245351
TGCACCT72200.017.6669061
GATGAAA50150.017.6221491
GCGCGAT45650.017.599341
CAATGGA66700.017.5794498
Produced by FastQC (version 0.11.5)