FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15035843
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG47650.019.4705831
GGAACTT47900.018.7256281
TACACGA58850.018.570221
TTGGAAC49450.018.561541
TTGAACG44950.018.4365461
TGACAAC51350.018.4105631
TCTAACT64250.018.3524788
CGCTATC63300.018.3079191
GAATGAA35600.018.2396411
GTAAATG58400.018.1755478
ATCGAGT51350.018.1319391
GAACCTA48550.017.9762131
CCGTGGA47400.017.9711951
CAATGGA53300.017.954168
TGACTAG46900.017.9281231
CGATTGA43150.017.8793411
TCTGGAA48350.017.8684751
ACGATGT65000.017.7952331
GACCTGG43700.017.729871
CCTACCA49300.017.7027441
Produced by FastQC (version 0.11.5)