FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15348175
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG48300.019.5282551
TTGGAAC49700.018.9560181
GGAACTT48450.018.8317411
GAATGAA38900.018.673431
GTAAATG55600.018.6555948
TCTAACT63300.018.575748
ATCGAGT55350.018.33341
TTGAACG43500.018.292741
TGACTAG45550.018.2909891
CGCTATC65650.018.273491
GACCTGG47000.017.9842831
GGCGACT45800.017.8787541
TACACGA62000.017.7161181
ACTGAAC41250.017.6896531
TGACAAC50750.017.674671
CACGAGT44200.017.6474133
TCTGGAA47400.017.646761
CAATGGA49450.017.594728
ACGATGT67000.017.5931821
CCGGGAT46650.017.576581
Produced by FastQC (version 0.11.5)