FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15288211
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG50200.019.2059731
GGAACTT51250.018.8769111
TTGGAAC49100.018.8516981
GTAAATG59250.018.3604158
ATCGAGT51650.018.283231
TTGAACG45050.018.2743851
TGACAAC52550.018.1795431
GACCTGG43900.018.1012541
CGCTATC60750.018.062791
ACGATGT67850.017.9894391
TGATTTC42700.017.9655651
TACACGA59250.017.9442251
TCTAACT62750.017.8797328
TGACTAG47450.017.860371
GAATGAA38950.017.8585241
ACTGAAC42750.017.8415721
TGCACCT53850.017.8224241
TCTGGAA45850.017.7394891
GAACCTA50500.017.6970391
CCGTGGA49000.017.564941
Produced by FastQC (version 0.11.5)