FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15288211
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC15194569.938742996155666No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC8870245.802013067454394No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC7865305.144683050227394No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC4456682.915108903193447No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC1544191.0100527785755966No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT1071680.7009845690905234No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC812910.5317234305570482No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG675460.4418175547158526No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT564990.3695592636705498No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT561110.3670213604456401No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA551360.3606438974449005No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA401110.2623655573565802No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTT383150.2506179434598332No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC365290.23893573944001686No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA363390.2376929517783343No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG354210.2316883250760995No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG340920.22299535243201443No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG321250.21012922963975314No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG318260.20817347431952632No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAA318170.20811460543028876No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC307580.2011876994633316No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTT304900.19943471476158983No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG301130.19696876240130387No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATG291990.19099029964984132No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGT287900.1883150356833772No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTC283500.18543700109842806No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTC279890.18307570454123115No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG278300.18203568749803362No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG270060.17664591363894702No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAA267580.1750237486910666No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGG257020.16811646568718866No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTG254640.16655971061623887No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTC248540.16256970812346846No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGA247680.16200718318186477No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTT235430.15399447325785862No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATTC231770.15160047176219638No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG218530.14294020405657668No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC218530.14294020405657668No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCAATC215380.14087979293326078No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG215040.14065739935169655No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCCGG201490.1317943610275918No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTGG186600.12205483035261613No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCAGC176870.11569044932726268No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGACTG167000.10923449447420631No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGA166770.10908405175726578No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGAATA166640.10899901891725593No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTAAGATCC166010.10858693669259276No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGACTTG165720.10839724804949383No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGACCACGTTTTTCAGGTCC160250.10481932778138658No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGGGTG158960.10397554036898103No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGAGAG155280.10156845689793265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTCT1538550.080.193251
TCTGATG1581500.078.700791
GTCTCTC1855500.066.5322652
TCTCTCA1906100.064.733593
CTCTCAG2263500.054.6608544
TCAGCTG2277950.054.1046337
AGCTGGT2280050.053.9243169
TCTCAGC2309750.053.550745
CAGCTGG2320300.053.16848
CTCAGCT2332200.052.9443136
CTGATGG4128950.051.9077031
TGATGGC4264250.050.3016972
TGATACG6500.048.3840033
GATGGCT4472200.047.7764973
TGGCTCA4517950.047.4134255
GGCTCAA4559000.047.160056
GCTCAAA4570050.046.9764337
ATGGCTC4548900.046.9757544
CTCAAAC4584850.046.85268
TCAAACA4617250.046.539479
Produced by FastQC (version 0.11.5)